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NEWS: A novel closed vitrification device reported to be effective to vitrify cleavage and blastocyst stage embryos

Open vitrification devices for embryo cryopreservation allow direct contact between embryo samples and liquid nitrogen, posing a risk of infection or cross-contamination between specimens. The advent of closed vitrification devices has circumvented these risks associated with open vitrification devices. A recent study highlights that Rapid-iTM, a new closed vitrification device, serves as an excellent alternative to the current open vitrification devices to cryopreserve embryos at the cleavage as well as the blastocyst stage.
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NEWS: Improved treatment outcomes reported with use of novel LandsonTM system to purify IVF laboratory air

Air quality in an in vitro fertilization (IVF) laboratory has been reported to play a vital role in determining treatment outcomes. Given the adverse impact of the presence of volatile organic compounds (VOCs), microbes, chemical air contaminants (CACs) etc, on in vitro embryo development, it is crucial for IVF laboratories to be equipped with an efficient, affordable, air filtration/purification system to eliminate airborne pathogens. A recent study, published in the Journal of Assisted Reproduction and Genetics, reports that a novel technology by LandsonTM series system to purify laboratory air significantly improves the ambient air and embryo quality, thereby enhancing pregnancy outcomes.
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NEWS: Low oxygen concentration for embryo culture reported to improve ART outcomes in a cleavage stage embryo transfer program

A recent time-series analysis, published in the journal Clinical Laboratory, reports that use of low oxygen concentration for embryo culture enhances the outcomes of assisted reproductive technology (ART) in a selective cleavage stage transfer program.

Federico Calzi and coworkers from the Obstetrics and Gynecology Unit, San Raffaele Scientific Institute, Milano, Italy, comparatively evaluated the effect of atmospheric and reduced oxygen concentration on the clinical and laboratory outcomes of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cycles.
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Study substantiates effectiveness of IMSI in couples with poor embryo development in prior ICSI cycles

A recent, comparative, prospective, randomized trial has concluded that the use of intracytoplasmic morphologically selected sperm injection (IMSI) technique improves embryo development, and clinical and laboratory outcomes of IVF in couples with male infertility and poor embryo development in their earlier ICSI attempts. Continue Reading

Posted in ART, Equipments, ICSI, IVF, IVF Outcome, Infertility, Male Infertility, TechnologyComments Off

New 20 Gauge Thin Tip Needle Reduces Pain During Oocyte Retrieval

Over the past two decades, ultrasound-guided transvaginal aspiration has emerged as the gold standard for oocyte retrieval in IVF. Pain experienced during the procedure is influenced by factors such as anxiety, type of analgesia, type and amount of additional IV analgesia, needle sharpness and diameter, pain threshold of the patient, and the doctor’s skills. A prospective, randomized, comparative, multi-centre study reports significant pain reduction, without any delay in the retrieval process or effect on retrieval rate with a newly designed, thinner-tipped reduced needle (RN) in contrast to the thicker standard needle (SN). Continue Reading

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Transabdominal Follicular Aspiration Effective for Oocyte Retrieval from Transvaginally Inaccessible Ovaries

A new retrospective case-control study has reported that oocyte retrieval via transabdominal ultrasound-guided follicular aspiration is safe and effective in IVF patients with substantial ovarian displacement, i.e. in cases where ovaries are inaccessible transvaginally. The study reported that the results were comparable to cycles where the oocytes were obtained by transvaginal aspiration in women with normally located ovaries. Continue Reading

Posted in ART, Diagnostics, Endometriosis, Equipments, Guidelines, ICSI, IVF, IVF Outcome, Infertility, TechnologyComments Off

NEWS: Determining Embryo’s Oxidative Status in Culture Media May Help Improve Implantation

Conventionally used measures to determine embryo quality, such as morphology and cleavage rate, have limited ability to predict successful outcome. A recent prospective laboratory study suggests that the evaluation of the oxidative status of an early embryo in the culture media may serve as a novel technique for improving embryo selection in IVF-ET cycles. The results of the study are published in the journal, Fertility and Sterility. Continue Reading

Posted in ART, Diagnostics, Embryology, Equipments, Female Infertility, Fertility, Fertility Preservation, IVF, IVF Outcome, Implantation, In-vitro Maturation, Infertility, Laboratory, Media and Consumables, News, Pregnancy, TechnologyComments Off

Brazilian Study Reports Similar Outcomes with ICSI and IMSI

Intracytoplasmic morphologically selected sperm injection (IMSI), a modification of ICSI procedure, evaluates motile sperm organellar morphology by magnifying the sample >6000 times with a high power microscope, enhanced by digital imaging. Several studies have demonstrated that IMSI, compared to ICSI, improves treatment outcomes, including the rate of implantation, pregnancy, and live birth. Now, a Brazilian research team reports similar outcomes, in terms of fertilization rate, embryo cleavage rate, and day 2 embryo quality, with both the conventional and modified ICSI procedures. However, the scientists did not rule out the possibility of IMSI positively influencing the late paternal effects. The study results have been published online in the European Journal of Obstetrics & Gynecology and Reproductive Biology. Continue Reading

Posted in ART, Embryology, Equipments, ICSI, IVF, IVF Outcome, Infertility, Male Infertility, TechnologyComments Off

Study Reiterates Efficacy of Long-term Liquid Nitrogen Storage for Preserving Sperm Motility

Study Reiterates Efficacy of Long-term Liquid Nitrogen Storage for Preserving Sperm Motility

Sperm banking has been recognized as an effective strategy to circumvent iatrogenic infertility and also retain the reproductive potential of adolescent and young adult males undergoing cancer therapy. However, the effect of long-term cryopreservation on the fertilization potential of sperm has been contentious. Now, a study performed by a group of Israeli researchers rules out any negative influence of prolonged storage in liquid nitrogen on the progressive motility concentration (PMC) of sperm. The results are published in the online issue of the journal Human Reproduction. Continue Reading

Posted in Cryopreservation, Embryology, Equipments, Featured, Fertility, Fertility Preservation, Infertility, Laboratory, Male Infertility, Media and Consumables, TechnologyComments Off

Vapor-phase Nitrogen System Superior to Traditional Liquid Nitrogen for Storing Vitrified Human Oocytes

Any therapeutic intervention in reproductive biology must ensure complete protection of biological materials against a wide range of microorganisms. Liquid nitrogen (LN) used for cryostorage collects particulate contaminants from the atmosphere or container surface over a period of time, which could be a potential source of pathogenic microbes, including herpes, papova, hepatitis, and vesicular stomatitis virus. A new study suggests that storage of vitrified oocytes using the vapor-phase nitrogen systems serve as a safer alternative to the traditional LN storage tank, as it avoids the risk of cross-contamination, apart from retaining the developmental potential of these oocytes. The findings of the prospective randomized trial have been published in the latest issue of Fertility and Sterility. Continue Reading

Posted in ART, Cryopreservation, Embryology, Equipments, IVF Outcome, Infertility, Laboratory, Media and Consumables, News, TechnologyComments Off


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