Lower embryo exposure reported with the use of laser for zona pellucida drilling in PGD cycles

A recent randomized study reports that using laser, instead of acid tyrode solution (ATZD), for zona pellucida drilling results in lesser embryo exposure during biopsy in preimplantation genetic diagnosis (PGD) cycles, and increases the availability of blastocysts for transfer and better treatment outcomes. Continue Reading

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NEWS: Rehybridization May Improve FISH Accuracy in Preimplantation Genetic Screening

A recent retrospective analysis, the findings of which were published in the journal, Human Reproduction, reports that performing additional rounds of hybridization during fluorescence in situ hybridization (FISH) improves the accuracy of the technique in preimplantation genetic aneuploidy screening (PGS), thereby increasing the number of competent embryos available for transfer. Continue Reading

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NEWS: Dutch Researchers Report MLPA Technique-based Blood Test for Prenatal Genetic Screening

A new breakthrough study has reported the development of a simple, non-invasive, prenatal blood test based on the multiplex ligation-dependent probe amplification (MLPA) technique, which could help in the accurate detection of chromosomal aberrations in the developing fetus, and thereby avoid the risk of miscarriage associated with conventional invasive techniques such as chorionic villus sampling and amniocentesis. The findings of the study were also presented recently at the 26th annual meeting of the European Society of Human Reproduction and Embryology (ESHRE), held at Rome from 27th-30th June, 2010. Continue Reading

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Novel Molecular PGD Technique Developed to Identify Chromosomal Translocations

Novel Molecular PGD Technique Developed to Identify Chromosomal Translocations

A team of researchers has developed a breakthrough embryo screening technique termed molecular preimplantation genetic diagnosis (PGD), which uses a PCR-based protocol for analyzing translocations. The new approach, having the potential to identify more viable embryos for transfer, utilizes multiplexed short tandem repeat (STR) markers that are positioned on both segments of the translocated chromosomes during translocation analysis. The findings of the study have been published in the journal, Molecular Human Reproduction. Continue Reading

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Case Series Findings Support PGD Safety in Singleton Pregnancies

In recent years, preimplantation genetic diagnosis (PGD) has emerged as a potential alternative to and compliments prenatal genetic diagnosis in preventing the birth of children with genetic/chromosomal abnormalities. Apart from congenital chromosomal and genetic abnormalities, PGD is currently used for the screening of late-onset diseases having a genetic predisposition, as well as preimplantation human leukocyte antigen (HLA) typing for the generation of savior siblings. However, certain researchers speculate that the technique is associated with adverse health effects including increased neurological defects in the offspring; probably due to the biopsy conducted in early stage embryos. Now, a recent prospective study published in the journal Human Reproduction rules out the risk for increased birth defects in singletons born after PGD. Continue Reading

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Multivariate Analysis Reports the Significance of Oocyte Donor Characteristics in Predicting IVF Success

Multiple factors have been attributed to affect the outcome of IVF pregnancies achieved through oocyte donation; some of them include embryo quality, endometrial receptivity, reproductive status of the recipient, and the ages of the donor and recipient. Despite contradictory evidences on the effect of the recipient’s age, the age of the donor has been identified as a significant factor influencing the outcomes of assisted reproduction. Results of a multivariate analysis published in the journal Fertility and Sterility has further substantiated the crucial association between the donor’s age and the number of mature oocytes/embryos obtained. The study results also proposed the significance of other factors, such as estradiol level on the day of hCG administration and amount of gonadotropin used in the stimulation protocol, for predicting IVF outcome. Continue Reading

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Study Reports Usefulness of PGS to Detect Genetic Abnormalities but Does Not Reveal the Full Chromosomal Constitution

Preimplantation genetic screening (PGS) is based on the hypothesis that the analysis of blastomeres extracted from a preimplantation embryo can represent the entire chromosomal constitution. Now, a recent randomized controlled study published in the journal Human Reproduction, highlights the potential of PGS in detecting genetically abnormal embryos, but the technique was not effective for evaluating their exact chromosomal constitution. Continue Reading

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Pre-implantation Genetic Diagnosis – Safety and Ethical Aspects

Pre-implantation genetic diagnosis (PGD) is one of the promising genetic and reproductive technologies that was developed during the mid 1980s in the United Kingdom, as an extension or alternative to prenatal diagnoses. With the technology opening up new avenues for genetic research, it offers hope to couples at a high risk of transmitting genetic disorders to their offspring, and also to those suffering from recurrent pregnancy loss and repetitive IVF failure. Continue Reading

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Study Reports Safety and Efficacy of eSET in PGD Cycles

The practice of elective single embryo transfer (eSET) in preimplantation genetic diagnosis (PGD) cycles is generally not followed due to the belief that embryo biopsy could affect its post-thaw survival rate. Now, a recent study has reported that eSET following PGD and freezing at blastocyst stage results in implantation and pregnancy rates similar to non-PGD cycles, thereby reducing the chances of multiple pregnancies. The results of the study were published online in the recent issue of Human Reproduction. Continue Reading

Posted in ART, Cryopreservation, Diagnostics, Embryology, Genetics, ICSI, IVF, IVF Outcome, PGDComments Off

ALERT: Novel Polar Body Chromosomal Analysis Test May Improve ART Prospects in Older Women

Aneuploidy in oocytes is known to be one of the main contributors to unsuccessful ART cycles, especially in women aged ≥38 years. Several factors, including maternal age, have been attributed to the occurrence of around 50% of the chromosomal aneuplodies. Researchers from the University of Oxford have now developed a new, less-invasive, genetic test for chromosomal analysis of polar bodies with comparative genomic hybridization (CGH), in order to improve the pregnancy rates in older women with multiple failed IVF cycles. The results of the study were presented at the 25th annual conference of the European Society of Human Reproduction and Embryology held at Amsterdam, from June 28 to July 1, 2009. Continue Reading

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